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<blockquote data-quote="BrockW" data-source="post: 441451" data-attributes="member: 5910">Sorry was watching football!No, you are correct. The protocol of RAMALT (rectal) via IHC is not USDA approved for regulatory purposes. IHC is an approved test. But RAMALT is not an approved tissue. But in my opinion, I think this is sort of a red herring argument. The lack of “approval” means relatively little. Test accuracy/sensitivity/specificity for that protocol it 93% sensitivity in wild type deer. Meaning it misses some positive deer due to lack of sensitivity, as shown in the table below.The reason RAMALT via IHC is not “approved” is because it’s not sensitive enough with 96S animals. Not that it’s working up positives out of thin air. 96S animals don’t accumulate prions as much or as quickly in RAMALT. Meaning those 96s animals can actually escape detection when IHC is used for that tissue, at about a 50% rate.[ATTACH=full]73628[/ATTACH]<a href="https://pmc.ncbi.nlm.nih.gov/articles/PMC2681868/" target="_blank">https://pmc.ncbi.nlm.nih.gov/articles/PMC2681868/</a></blockquote>

[QUOTE="BrockW, post: 441451, member: 5910"] Sorry was watching football! No, you are correct. The protocol of RAMALT (rectal) via IHC is not USDA approved [U]for regulatory purposes[/U]. IHC is an approved test. But RAMALT is not an approved tissue. But in my opinion, I think this is sort of a red herring argument. The lack of “approval” means relatively little. Test accuracy/sensitivity/specificity for that protocol it 93% sensitivity in wild type deer. Meaning it misses some positive deer due to lack of sensitivity, as shown in the table below. The reason RAMALT via IHC is not “approved” is because it’s not sensitive enough with 96S animals. Not that it’s working up positives out of thin air. 96S animals don’t accumulate prions as much or as quickly in RAMALT. Meaning those 96s animals can actually escape detection when IHC is used for that tissue, at about a 50% rate. [ATTACH type="full"]73628[/ATTACH] [URL]https://pmc.ncbi.nlm.nih.gov/articles/PMC2681868/[/URL] [/QUOTE]

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